진학프로석·박사 채용 접수 플랫폼

The University of Bordeaux is a great dynamic and responsible university that cares about the well-being of its staff. Joining us means working in a privileged environment within a particularly diverse and open professional community, benefiting from welcome and inclusion schemes, training and internal mobility. It means participating in an academic, scientific and human adventure. It means committing to meeting the challenges of the 21st century.

Join the Institute of Molecular Sciences (ISM) – of the University of Bordeaux!

ISM brings together a community of organic and physical-chemical researchers interested in molecular structures, and working on their design, synthesis, characterization, reactivity and analysis in various environments. The Institute's expertise is based on a number of key skills: theorists in quantum chemistry, molecular and reaction dynamics, synthetic organic chemists, physical-chemical experimentalists (spectroscopy, photochemistry, molecular recognition and analysis), working in 10 research groups and 3 technical platforms.

As part of the Interdisciplinary and Exploratory Research (RIE) project GlycoMechano – Crosslinking of glycans to modulate the mechanical properties of the brain, we are recruiting a Post-Doctoral W/M whose work will focus on biological chemistry for the synthesis and imaging of non-natural sugars.

The aim of the GlycoMechano project is to develop molecular tools capable of modulating the rigidity of the neural extracellular matrix (ECM) of a neuronal tissue in order to study the effect of a rigidification of the synaptic environment on plasticity.

Indeed, the molecular mechanisms of synaptic plasticity most studied are by far biochemical. However, the synapses are very motile and are surrounded by a dense and complex extracellular matrix. They are therefore subject to numerous mechanical constraints. The objective of the project will be to allow the visualization and manipulation of glycan chains of the extracellular matrix by metabolic labeling of unnatural oligosaccharides in neuronal tissues in order to study the effect of mechanical constraints on synaptic physiology.

Main activities:

Your mission will be to develop molecular tools and the cell labeling strategy from organic sugar synthesis to their visualization by fluorescence microscopy at various levels of resolution, including their validation in different cellular models. You will have to regularly present your research results (oral presentations to both teams, writing reports and publications) and carry out bibliographic monitoring on the subject.

# Organic synthesis

• • You perform syntheses, purification and characterization of non-natural sugars for metabolic labeling
• • You carry out the synthesis, purification and characterization of bioorthogonal probes for imaging

# Cell culture and microscopy

• • You produce immortalized lines, dissociated neurons on mouse models and tissues, notably brain slices
• • You carry out fluorescence imaging shots: epifluorescence, confocal, dSTORM, DNA-PAINT, sptPALM ...
• • You analyze the images acquired with the software Fiji, Imaris, Matlab, ...

# Life of the laboratory and scientific dissemination

• • You prepare and host oral and/or written presentations of research findings at team meetings, institute meetings, activity reports, publications, conferences
• • You conduct a bibliographic monitoring
• • You contribute to the collective tasks of the laboratory: logistics, ...