진학프로석·박사 채용 접수 플랫폼

1227.1101.26.2026

RECTOR of the Jagiellonian University announces a selection procedure for the position of an ASSISTANT PROFESSOR

Project Title: OPUS 27 Characterization of nucleic acids as the allosteric activators of PAD4, PSP: K/NCN/000478

Project description

The selected candidate will work on the NCN-funded project aimed at investigating the interactions between human peptidylarginine deiminase 4 (PAD4) and various types of nucleic acids. PAD4 is an enzyme whose activity is regulated by calcium ion binding. However, the relatively low physiological concentrations of these ions in the human body, which are insufficient for full enzyme activation, suggest the existence of other regulatory mechanisms. Due to its nuclear localization signal and involvement in nucleic acid-related processes (such as NETosis), we hypothesize that PAD4 can be activated by DNA/RNA. The selected candidate will be involved in elucidating the detailed mechanism of PAD4 interaction and activation by nucleic acids, both in the context of biochemical and cellular studies.

Field of science: Science and life sciences

Discipline: Biological sciences

Scope: Biochemistry / cell biology

Planned duration of employment : 34 months

Expected date of employment commencement: I/II quarter 2026

Remuneration

• • basic remuneration in the range of 7 395,00‒8 870,00 PLN gross;
• • seniority allowance depending on length of service (3‒20%);
• • jubilee awards for long-term service ‒ in accordance with the Regulation for remuneration of employees of the Jagiellonian University;
• • possible task-based and periodic allowances, as well as discretionary awards, upon meeting the conditions set out in the Regulation for remuneration of employees of the Jagiellonian University;
• • additional annual remuneration, the so-called "thirteenth salary" ‒ after meeting the conditions specified in the relevant regulation

Benefits ‒ Institutional Social Benefits Fund (depending on the income per family member, in accordance with the Regulations for Granting Benefits from the Company Social Benefits Fund of the Jagiellonian University):

• • co-financing of summer holidays (PLN 1050–1550 gross) and winter holidays (PLN 700–900 gross);
• • co-financing for children for organised holidays (PLN 900–1400 gross), or co-financing of individually purchased summer camps, sports camps, winter camps, medical holidays or rehabilitation stays (50–90%, with the maximum amount eligible for co-financing being PLN 2500 gross) per child;
• • cash equivalent for a special occasional package of sweets in the amount of PLN 100–300 gross per child;

co-financing of childcare in nurseries, kindergartens and other forms of preschool education, as well as care provided by a daily guardian or nanny (PLN 250–400 gross).

Scope of duties

according to the Work Regulations of the Jagiellonian University Annex 1 to the Work Regulations of the Jagiellonian University – Model scopes of responsibilities and duties of academic teachers (F/M)

The Candidate's (F/M) responsibilities will include:

• • Designing and preparing constructs for the expression of PAD4 protein variants.
• • Purifying recombinant proteins using liquid chromatography techniques.
• • Analyzing the activity, structure, and function of PAD4 protein variants in the context of interactions with various nucleic acids (activity assays, SPR, MST, thermal stability, tryptophan residue fluorescence).
• • Participating in crystallographic/cryo-EM studies of PAD4-nucleic acid complexes.
• • Contributing to research on the identification of selective aptamers for PAD4 activation/inhibition.
• • Assisting in the preparation of samples for proteomic analysis of cell lines with different PAD4 variants.