진학프로석·박사 채용 접수 플랫폼

The Institute of Biochemistry and Biophysics of the Polish Academy of Sciences is one of the leading research institutes in the field of life sciences in Poland. The groups of IBB carry out basic research in various areas of biology, biophysics, biochemistry, genetics, and Antarctic biology. TheLaboratory of Bioenergetics and Mitochondrial Disease Mechanisms focuses its research on mitochondrial ATP synthase, its regulation, biogenesis, and deficiencies. We seek a highly motivated Postdoc candidate to join a group under the supervision of Prof. Roza Kucharczyk. 

Project background

AMPylation and depalmitoylation in regulating mitochondrial energy production and redox balance 

Oxidative phosphorylation (OXPHOS) in mitochondria is a key cellular process. It not only provides ATP, the main energy currency required for basic metabolic reactions, but also influences mitochondrial physiology and overall cellular function. Respiratory chain complexes and ATP synthase operate in a coordinated and interdependent manner; however, the mechanisms underlying this regulation remain poorly understood. In addition, OXPHOS activity is associated with the production of reactive oxygen species (ROS), which serve important signaling functions. As a result, OXPHOS directly affects cellular redox homeostasis and ROS-mediated signaling pathways.

This project aims to investigate the roles and mechanisms of two mitochondria-localized enzymes, AMPylase Fmp40 and depalmitoylase Abh1, in regulating oxidative phosphorylation (OXPHOS) and maintaining redox homeostasis within mitochondria. In this project, we focus on how these two post-translational modifications regulate mitochondrial ATP synthase. Visit our website to learn more about us: https://ibb.edu.pl/en/laboratory/roza-kucharczyk/#research

Job description

The Postdoctoral researcher will join a six-person group of three experienced scientists and three doctoral students.

Tasks planned for the post-doc are aimed at and include:

• • Study of global effects of Fmp40 on protein oxidation and cellular metabolome: i) large-scale proteomic analysis to assess protein oligomerization and complex formation via S–S bridges, and ii) large-scale analysis of cellular metabolome changes upon oxidative stress in cells lacking Fmp40 ampylase.
• • Establishment of workflows using the proximity-labeling TurboID method and screening of Fmp40 interactome and a nucleotide-binding protein HinT-based approach to pull down AMPylated proteins by Fmp40.
• • Design and implementation of experiments aiming to study Fmp40 regulation.
• • Understanding the regulation of ATP synthase activity by ampylation and phosphorylation - mitochondrial bioenergetics study on native mitochondria, establishment of Phos-tag method for study of protein ampylation.